molecular and pathological study of bovine aborted fetuses and placenta from neospora caninum infected dairy cattle

Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR.

We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA fragment corresponding to the Nc5 sequence of N. caninum. A similar method was developed to quantify ...

Bacillus anthracis isolated from aborted bovine fetuses

Estimating the rate of transplacental transmission of Neospora caninum to aborted fetuses in seropositive dams in Mashhad area, Iran

BACKGROUND: Neospora caninum is an apicomplexan protozoa,which causes abortion in cattle worldwide. OBJECTIVES:The present study was designed to estimate the rate oftransplacental transmission of N.caninum to aborted fetuses inseropositive dams in dairy farms in Mashhad area, Iran.METHODS: Two hundred blood samples of dam with abortedbovine fetuses were collected from dairy farms from 2008 to20...

Molecular detection of Neospora caninum from naturally infected dogs in Lorestan province, West of Iran

Neospora caninum is a coccidian protozoan that causes abortion in dairy and beef cattle and neurological disorders in dogs and horses. To identify N. caninum oocysts in the dog feces the molecular approaches are known as sensitive methods that specifically detect the oocysts. In present study, a polymerase chain reaction (PCR) targeting N. caninum specific Nc5 genomic fragment was performed to ...

Molecular detection of Neospora caninum abortion in dairy cattle from different historical regions of Romania

Neosporosis is a major cause of abortion in many countries, transplacental transmission being considered the major route of transmission of N. caninum in cattle. Little information are available about the prevalence of N. caninum abortion in dairy cattle in Romania, the only existing data are coming from Transylvania region. In the present study, we analyzed by molecular technique (PCR) the pre...

Prevalence of Neospora caninum and Toxoplasma gondii Antibodies in Bulk Milk of Dairy Cattle, Mashhad, Iran

Neospora caninum (N. caninum) and Toxoplasma gondii (T. gondii) are both obligate intracellular protozoan parasites, which have gained considerable attention because of their role in bovine abortion. This study aimed to detect anti-N. caninum and -T. gondii in bulk milk of dairy cattle, Mashhad, Iran. The bulk milk samples were collected from July 2014 to June 2015 and analyzed for anti-N. cani...